18 records found...
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An In-house Method for Molecular Monitoring of BCR-ABL
Figure 3: Dilutions of K562 cells were prepared in the background of the BCR-ABL-negative RS4;11 cell line and were subjected to RQ-PCR. Previously determined standard curves were used to calculate the BCR-ABL and ABL transcript copy numbers, and the results are plotted on a logarithmic scale (Y-axis). Change/reduction in the BCR-ABL transcript level is expressed as the ratio of BCR-ABL / ABL.
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Variant Philadelphia translocations with different breakpoints in six chronic myeloid leukemia patients
Figure 2. FISH analysis results. A. Signals of BCR-ABL fusion on interphase cells via the BCR-ABL1 D-FISH probe (ONCOR). Green signals on 1 of chromosome 9, red signals on 1 of chromosome 22, and yellow signals on the Ph chromosome showing BCR-ABL fusion. B. In patient 2 metaphase FISH showing t(9;22;15) via WCP 15 (ONCOR) red signals. C. In patient 4 metaphase FISH showing t(1;9;22;3) via WCP 1, 9, and 22 (MetaSystems). CI. Chromosome 1 is green and chromosome 22 is red. CII. and chhromosome 9 is green and chromosome 22 is red. D. In patient 6 metaphase FISH showing t(4;8;9;22) via WCP 4 and 8, (Cytocell), and WCP 9 and 22 (MetaSystems). DI. Chromosome 4 is green and chromosome 8 is red. DII. Chromosome 4 is green and chromosome 22 is red. DIII. Chromosome 8 is red and chromosome 9 is green
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